StarBright Violet 515 Dye from Bio-Rad Enhances Flow Cytometry

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In the Lab eNewsletterPharmaceutical Technology's In the Lab eNewsletter 10-07-2020
Volume 15
Issue 10

The SBV515 Dye from Bio-Rad improves brightness to enhance its use in flow cytometry.

The StarBright Violet 515 (SBV515) Dye, a range of fluorescent nanoparticles from Bio-Rad Laboratories, can enhance flow cytometry. With an excitation maximum at 401 nanometers and emission maximum of 516 nanometers, the dye improves brightness to solve rare and low antigen density populations. In addition, its narrow emission profile reduces spillover onto other filters and allows only minimal excitation by other lasers.

The dye is not vulnerable to photobleaching, distributes high lot-to-lot reproducibility, and is stable at 4° C with no loss of signal. There is also no requirement to use a special staining buffer, which allows for integration into common experimental protocols without compromising performance.

“StarBright Violet 515 Dye is brighter than other common dyes that emit at similar wavelengths,” said Mike Blundell, Bio-Rad product manager, Life Science Group, in a Sept. 14, 2020 press release. “The dye is flexible enough to fit into any flow cytometry panel, allowing researchers to multiplex with ease regardless of flow cytometer and experimental protocol, and it offers a superior alternative for fluorophores that have emission maxima between 500 and 550 nanometers.”

Source: Bio-Rad​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​

Articles in this issue
Restructuring Method Transfer Throughout Global Sites
Restructuring Method Transfer Throughout Global Sites
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When Assessing Protein Aggregation Grows Increasingly Challenging
Almac Sciences Adds Biologics Testing to its Analytical Solutions Suite
PharmaLex Group Completes Merger with BioStat Solutions
Natoli Installs Tablet Press at its New Research and Training Facility
Thermo Fisher to Invest More than $140 Million into its Lab Plastics Consumables for COVID-19 Testing
Sai Life Sciences’ Screening Capabilities Enhanced with Expanded Cellular Analysis Platforms
StarBright Violet 515 Dye from Bio-Rad Enhances Flow Cytometry
Paradigm4’s Single Cell App Allows for Analysis of Large-Scale Datasets
CytoSMART Technologies’ New Automated Live-Cell Imaging System Keeps Lab Teams Coordinated
Promega’s New Capillary Electrophoresis Instrument Enhances Benchtop Analysis
Aigenpulse’s Data Analysis Suite Automates Flow Cytometry
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